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Protocol Protocol for the labeling of biomolecules containing primary amino groups with NHS esters

Product category Bifunctional activated NHS and STP esters for binding with primary and secondary amine groups.

Product Dicyano BDP 630/650 is a far-red fluorescent dye from the boron dipyrromethene (BDP) family, known for its high brightness and exceptional photostability. Its remarkable stability in strong acidic TFA media makes it particularly suitable for solid-phase peptide synthesis (SPPS). BDPep 630/650 NHS ester can be used for effective cell sorting and phenotyping.
Cat. # 1M420, 2M420, 4M420, 5M420, 6M420

Product Biotin derivative with C6-spacer to reduce steric effect when binding amino acids, peptides, or proteins by reacting with primary amines.
Cat. # 2551-100mg, 2551-1g

Product BDP TR is a borondipyrromethene dye. A bright and photostable fluorophore with high quantum yield.
Cat. # 13420, 23420, 43420, 53420, 63420

Product Homobifunctional crosslinker with two NHS ester groups at each end of a short 5-atom (7.7 angstrom) spacer.
Cat. # 4018-100mg, 4018-1g

Product BDP FL analog derivative suitable for solid phase synthesis SPPS.
Cat. # 2J420, 4J420, 5J420, 6J420

Product BDP TR methyl ester is a lipophilic counterstain for GFP. Сell-permeable dye that stains mitochondria and endomembranous organelles, but not plasma membrane.
Cat. # 1171-1mL

89

What are hydrolysis rates for NHS ester functionalized dyes?


Help NHS ester tends to hydrolyze over time in the water.


90

What is the color of sulfo-Cyanine7.5 NHS ester after dissolving in water and in DMF?


Help The color of sulfo-Cyanine7.5 NHS ester in water and in DMF solutions.


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