AF 430 hydrazide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 10870 | 1 mg | $110 | in stock | |
| 20870 | 5 mg | $210 | in stock | |
| 40870 | 25 mg |
$410
|
in stock | |
| 50870 | 50 mg |
$695
|
in stock | |
| 60870 | 100 mg |
$1190
|
in stock |
AF 430 is a fluorescent dye with an excitation maximum at 430 nm and an emission maximum at 542 nm. AF 430 is one of the few dyes that absorb between 400 nm and 450 nm. AF 430 fluorescence is photostable and pH-insensitive in a broad range of pH values.
This product is a hydrazide derivative of AF 430 dye. Hydrazides efficiently react with aldehydes and ketones, resulting in hydrazones, so that this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with hydrazides). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can also be conjugated with hydrazides in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
Absorption and emission spectra of AF 430
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AF 430 maleimide
AF 430 is a green-yellow emitting fluorescent dye of the coumarin series. This maleimide derivative allows the labeling of thiolated biomolecules.(5,6)-FAM diacetate NHS ester
Amine-reactive fluorescein (FAM) derivative, a mixture of 5- and 6-isomers. The reagent becomes brightly green fluorescent once it is hydrolyzed while diffusing into cells. A fluorescent tracer.AF 430 NHS ester
AF 430 is a hydrophilic coumarin dye with emission in the green-yellow range. The NHS ester is used for the labeling of amine groups.General properties
| Appearance: | yellow powder |
| Mass spec M+ increment: | 499.1 |
| Molecular weight: | 517.52 |
| Molecular formula: | C22H26N3F3O6S |
| Solubility: | good in DMF, DMSO, water |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
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