AF 647 TCO
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1G8H0 | 1 mg |
$196.00
|
in stock | |
| 2G8H0 | 5 mg |
$528.00
|
in stock | |
| 4G8H0 | 25 mg |
$1680.00
|
in stock |
AF 647 TCO is a derivative of the AF 647 fluorophore functionalized with trans-cyclooctene (TCO) for rapid and highly selective bioorthogonal conjugation to tetrazine-containing molecules.
The dye belongs to the far-red spectral region and is characterized by high molar absorptivity, high quantum yield, and excellent photostability. AF 647 TCO delivers a bright fluorescent signal with excitation and emission maxima at 655/680 nm, making it fully compatible with standard 633–640 nm laser lines. Its emission is well separated from the yellow—green region and shows minimal overlap with tissue autofluorescence.
The TCO moiety undergoes a rapid inverse electron-demand Diels—Alder (IEDDA) reaction with tetrazines, enabling site-specific labeling of proteins and antibodies, conjugation to oligonucleotides and other biomolecules, as well as metabolic labeling in live cells.
Owing to the fast reaction kinetics and excellent biocompatibility of the IEDDA approach, AF 647 TCO is suitable for in vitro and in cellulo applications, including flow cytometry, widefield fluorescence microscopy, confocal microscopy, and super-resolution imaging.
Absorption and emission spectra of AF 647
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General properties
| Appearance: | deep-blue powder |
| Molecular weight: | 1209.66 |
| Molecular formula: | C50H67K3N4O15S4 |
| Solubility: | water, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 655 |
| ε, L⋅mol−1⋅cm−1: | 191800 |
| Emission maximum, nm: | 680 |
| Fluorescence quantum yield: | 0.15 |
| CF260: | 0.09 |
| CF280: | 0.08 |
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