Alkyne phosphoramidite, 5'-terminal
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 22260 | 250 mg | $110 | in stock | |
| 32260 | 500 mg | $210 | in stock | |
| 42260 | 1 g | $350 | in stock | |
| 62260 | 5 g |
$1290
|
in stock | |
| 82260 | 10 g | please inquire | in stock |
Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for click chemistry.
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life
Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5'-terminal DMT group, no 5'-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.
Oligonucleotides with this modification are ideal for the use in click chemistry (see our recommended protocol).
Customers also purchased with this product
AF 647 azide
Bright and photostable azide AF 647 for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects.ROX azide, 5-isomer
Azide derivative of ROX (Rhodamine X, Rhodamine 101) dye for biomolecule labeling via click chemistry. Pure 5-isomer.TFA-amino modifier CPG 500
TFA-amino modifier CPG for oligo synthesis. 500 Å, high load controlled pore glass.General properties
| Appearance: | colorless solid |
| Molecular weight: | 409.50 |
| CAS number: | 1417539-32-2 |
| Molecular formula: | C21H36N3O3P |
| IUPAC name: | trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine |
| Solubility: | good in acetonitrile and dichloromethane |
| Quality control: | NMR 1H (95%) and 31P, HPLC-MS |
| Storage conditions: | Storage: 12 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | Standard coupling, identical to normal nucleobases. |
| Cleavage conditions: | standard deprotection |
| Deprotection conditions: | No deprotection required. Compatible with standard deprotection reagents. |
Product citations
- Farzan, V.M.; Kvach, M.V.; Aparin, I.O.; Kireev, D.E.; Prikazchikova, T.A.; Ustinov, A.V.; Shmanai, V.V.; Shipulin, G.A.; Korshun, V.A.; Zatsepin, T.S. Novel homo Yin-Yang probes improve sensitivity in RT-qPCR detection of low copy HIV RNA. Talanta, 2019, 194, 226–232. doi: 10.1016/j.talanta.2018.10.043
- Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
- Aparin, I.O.; Farzan, V.M.; Veselova, O.A.; Chistov, A.A.; Podkolzin, A.T.; Ustinov, A.V.; Shipulin, G.A.; Formanovsky, A.A.; Korshun, V.A.; Zatsepin, T.S. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst, 2016, 141, 1331–1338. doi: 10.1039/c5an01767j
- Astakhova, I.K.; Santhosh Kumar, T.; Campbell, M.A.; Ustinov, A.V.; Korshun, V.A.; Wengel, J. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-alpha-L-amino-LNA FRET pairs. Chemical Communications, 2013, 49(5), 511-511. doi: 10.1039/c2cc37547h
Your cart is empty
$ 
