DusQ 1 phosphoramidite
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 1470-100mg | 100 mg | $290 | in stock | |
| 1470-500mg | 500 mg |
$990
|
in stock | |
| 1470-1g | 1 g |
$1690
|
in stock | |
| 1470-10g | 10 g | please inquire | in stock |
DusQ 1 phosphoramidite is a true dark quencher with broad absorption curve which covers the visible spectrum with maximum in green to yellow region. It is used for the synthesis of dual labeled oligonucleotide probes for qPCR bearing 5'-quencher DusQ 1 and other FRET applications for multiplexing assays. Contains a DMT protection of the hydroxymethyl group, which allows oligonucleotide purification on cartridges.
DusQ 1 has a quenching range (QR) 480 - 580 nm to construct efficiently quenched qPCR probes paired with all common reporter dyes such as FAM, TET, JOE, HEX and Cyanine3 with high quenching efficiency, and form completely non-fluorescent complexes.
Usage
Coupling: 6 minutes coupling time recommended.
Deprotection: for 2 h at RT using ammonium hydroxide, or 10 min at 65 °C with AMA (solution of 30% ammonium hydroxide/40% aqueous methylamine 1:1 v/v).
Deprotection time depends on oligonucleotide composition and nucleobase protecting groups, and additional modifications.
Absorption spectrum of DusQ 1
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DusQ 1 amidite, 5'-terminal
Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ 1 quencher.General properties
| Appearance: | black powder |
| Molecular weight: | 995.11 |
| CAS number: | 374591-94-3 |
| Molecular formula: | C55H63N8O8P |
| IUPAC name: | Phosphoramidous acid, bis(1-methylethyl)-, 2-[[2-[bis(4-methoxyphenyl)phenylmethoxy]ethyl][4-[[2-methoxy-5-methyl-4-[(4-methyl-2-nitrophenyl)azo]phenyl]azo]phenyl]amino]ethyl 2-cyanoethyl ester (9CI) |
| Solubility: | good in acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
Oligo synthesis details
| Diluent: | 50% DCM in acetonitrile |
| Coupling conditions: | 6 min coupling time; 3 min oxidation time |
| Cleavage conditions: | ammonia, 2 h at room temperature |
| Deprotection conditions: | identical to protected nucleobases |
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