Bis-ANS, protein conformation probe
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 4381-5mg | 5 mg |
$159.00
|
in stock | |
| 4381-25mg | 25 mg |
$397.00
|
in stock |
Bis-ANS is a high-affinity fluorescent probe for nonpolar cavities in proteins. Its hydrophobic phenyl and naphthyl rings interact noncovalently with proteins and protein degradation products.
As with other anilinonaphthalene sulfonates (ANS), bis-ANS is essentially nonfluorescent in water but becomes noticeably fluorescent in a nonpolar environment. When free, bis-ANS has an excitation maximum at 390 nm and an emission maximum at 523 nm but undergoes a blue shift with an increase in fluorescence intensity when bound to protein.
Bis-ANS is frequently used to monitor the formation of protein aggregates and indicate protein folding and conformational changes. bis-ANS binds to tubulin and can be used for cell visualization[1]. The dye is also used to detect Aβ fibers[2].
[1] Brain Res. Bulletin. 2011, 86, 3–4. 217-221; [2] Biochemistry. 2015, 54, 28, 4297–4306.
Excitation and emission spectra of protein complex with Bis-ANS
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General properties
| Appearance: | light yellow-green crystals |
| Molecular weight: | 672.87 |
| CAS number: | 65664-81-5 |
| Molecular formula: | C32H22K2N2O6S2 |
| IUPAC name: | dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate |
| Solubility: | DMF: 30 mg/ml, DMSO: 30 mg/ml, ethanol: slightly soluble, PBS (pH 7.2): 5 mg/ml |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 396 (free), 401 (complex) |
| Emission maximum, nm: | 508 (free), 488 (complex) |
Product citations
- Sedlov, I. A.; Sluchanko, N. N. Biochemical Characterization of Selaginella 14-3-3 Protein Isoforms Provides Insights into the Evolution of Their Phylogenetic Groups. Plant Physiology and Biochemistry, 2025, 227, 110197. doi: 10.1016/j.plaphy.2025.110197
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