SynaptoProbe® Red
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3425-100ug | 100 ug | – | in stock | |
| 3425-1mg | 1 mg | $132 | in stock | |
| 3425-5mg | 5 mg | $350 | in stock |
SynaptoProbe® Red is an analog of FM®4-64, a widely used red fluorescent dye for studying synaptic activity in the synapses or neuromuscular junctions by staining synaptic vesicles.
This water-soluble and non-toxic to cells dye is nonfluorescent in an aqueous medium but becomes highly fluorescent after embedding into the outer leaflet of the cell membrane. When a neuron actively releases neurotransmitters, the dye becomes internalized within the recycled synaptic vesicles and stains the nerve terminals.
SynaptoProbe® Red can be used to visualize synaptic vesicles and their exocytosis and endocytosis in living neurons, identify actively firing neurons, and investigate the mechanisms of activity-dependent vesicle cycling. It is also helpful for the visualization of shear stress-induced plasma membrane damage in fibroblasts.
Absorption and emission spectra of SynaptoProbe Red
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FAM alkyne, 5-isomer
FAM alkyne, 5-isomer - an alkyne derivative of fluorescein dye for click chemistry.General properties
| Appearance: | dark powder |
| Molecular weight: | 700.51 |
| CAS number: | 162112-35-8 (bromide salt) |
| IUPAC name: | 4-{6-[4-(diethylamino)phenyl]hexa-1,3,5-trien-1-yl}-1-[3-(triethylammonio)propyl]pyridinium diiodide |
| Solubility: | good in water |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 536 |
| Emission maximum, nm: | 736 |
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