LUCS® 9, green fluorescent nucleic acid stain
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2M010 | 100 uL, 5 mM/DMSO |
$179
|
in stock |
LUCS 9 is a cell-permeant nucleic acid stain that exhibits green fluorescence upon binding to nucleic acids. The stain has a high fluorescent yield and a structure identical to SYTO™9 stain.
LUCS 9 is used to stain both DNA and RNA in live and dead eukaryotic cells as well as Gram-positive and Gram-negative bacteria. The dye is excited by the blue laser at 485 nm. Its fluorescence emission is detected in the fluorescein channel with a peak at 500 nm when bound to DNA and 504 nm when bound to RNA.
The dye can be used in simultaneous labeling with cell-impermeant nuclear markers, such as YoDi-3, propidium iodide, or 7-AAD to evaluate cell viability using fluorescence microscopy and flow cytometry.
LUCS 9 is especially useful as a counterstain for bacterial assays due to its ability to stain both live and dead Gram-negative and Gram-positive bacteria.
Excitation and emission spectra of dsDNA complex with LUCS® 9
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General properties
| Appearance: | orange solution |
| Molecular weight: | 534.44 |
| Molecular formula: | C28H27IN2O |
| Solubility: | miscible with water |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 477 (free), 482 (DNA complex) |
| Emission maximum, nm: | 500 (DNA complex), 504 (RNA complex) |
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