Cyanine5.5 alkyne

Cyanine5.5 Alkynes Click chemistry
Cat. # Quantity Price Lead time
A70B0 1 mg $125 in stock
B70B0 5 mg $260 in stock
C70B0 10 mg $325 in stock
D70B0 25 mg $510 in stock
E70B0 50 mg $895 in stock
F70B0 100 mg $1490 in stock
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Far red / near-infrared dye alkyne for сlick сhemistry labeling. Cyanine5.5 is an analog of Cy5.5®, a popular fluorophore widely used for various applications, including intact organism imaging. This reagent can be conjugated with azido groups under mild copper-catalyzed сlick сhemistry conditions.

This reagent is soluble in organic solvents, but mixtures of water with a small percent of DMSO can be used for efficient conjugation. Cyanine5.5 alkyne can also be used for the labeling of small molecules with this far red/NIR dye.

Cyanine5.5 alkyne, F70B0
Cyanine5.5 alkyne, F70B0
Cyanine5.5 alkyne, F70B0
Structure of Cyanine5.5 alkyne
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Absorbance and emission spectra of Cyanine5.5

Absorbance and emission spectra of Cyanine5.5

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Fluorescein (FAM) maleimide is a thiol reactive dye derived from isomerically pure 6-FAM. Fluorescein is a bright dye that is compatible with various fluorescence measurement instruments.

DMF (dimethylformamide), labeling grade

Ready-to-use high purity, amine-free solvent for NHS ester labeling.

General properties

Appearance: dark blue powder
Molecular weight: 656.30
CAS number: 1628790-37-3
Molecular formula: C43H46ClN3O
Solubility: good in organic solvents (DMF, DMSO, acetonitrile, DCM, alcohols), practically insoluble in water (5.2 uM, 3.7 mg/L)
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: Download
Product specifications

Spectral properties

Excitation/absorption maximum, nm: 684
ε, L⋅mol−1⋅cm−1: 198000
Emission maximum, nm: 710
Fluorescence quantum yield: 0.2
CF260: 0.07
CF280: 0.03

Product citations

  1. Agnes, R.S.; Traughber, B.J.; Muzic, R.F. Development of a selective novel fluorescent substrate for sodium-dependent transporters. Life sciences, 2024, 351, 122847. doi: 10.1016/j.lfs.2024.122847
  2. Arranz-Gibert, P.; Vanderschuren, K.; Haimovich, A.; Halder, A.; Gupta, K.; Rinehart, J.; Isaacs, F. J. Chemoselective Restoration of Para-Azido-Phenylalanine at Multiple Sites in Proteins. Cell Chemical Biology, 2022, 29(6), 1046-1052.e4. doi: 10.1016/j.chembiol.2021.12.002
  3. Li, M.; Ruwe, H.; Melzer, M.; Junker, A.; Hensel, G.; Tschiersch, H.; Schwenkert, S.; Chamas, S.; Schmitz-Linneweber, C.; Börner, T.; Stein, N. The Arabidopsis AAC Proteins CIL and CIA2 Are Sub-functionalized Paralogs Involved in Chloroplast Development. Frontiers in Plant Science, 2021, 12, 681375. doi: 10.3389/fpls.2021.681375
  4. Li, M.; Hensel, G.; Melzer, M.; Junker, A.; Tschiersch, H.; Ruwe, H.; Arend, D.; Kumlehn, J.; Börner, T.; Stein, N. Mutation of the ALBOSTRIANS Ohnologous Gene HvCMF3 Impairs Chloroplast Development and Thylakoid Architecture in Barley. Frontiers in Plant Science, 2021, 12, 732608. doi: 10.3389/fpls.2021.732608
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Cy® is a trademark of GE Healthcare.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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