5-Ethynyl-uridine (EU)
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 2439-25mg | 25 mg | $137 | in stock | |
| 2439-100mg | 100 mg |
$487
|
in stock | |
| 2439-500mg | 500 mg |
$950
|
in stock |
5-Ethynyl-uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.
EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyluridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.
EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via click chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.
5-Ethynyl-uridine has an advantage over its analog, 5-bromo-uridine, because azide-containing dyes are very small in size and exhibit better membrane permeability compared to antibodies used for the detection of 5-bromo-uridine. Thus, the click chemistry approach allows whole-mount staining of large samples like organs or tissue fragments.
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Ready-to-use catalytic buffer containing сopper(II) and water-soluble THPTA ligand. It is suitable for click chemistry modification of proteins.EdU (5-Ethynyl-2'-deoxyuridine)
EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.General properties
| Appearance: | off white solid |
| Molecular weight: | 268.22 |
| CAS number: | 69075-42-9 |
| Molecular formula: | C11H12N2O6 |
| IUPAC name: | 1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione |
| Solubility: | good in water, DMSO, DMF |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | Download |
| Product specifications |
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