FAM hydrazide, 5-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 14170 | 1 mg | $110 | in stock | |
| 24170 | 5 mg | $210 | in stock | |
| 44170 | 25 mg |
$410
|
in stock | |
| 54170 | 50 mg |
$695
|
in stock | |
| 64170 | 100 mg |
$1190
|
in stock |
Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.
Compounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl compounds for the subsequent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.
FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl compounds.
Absorption and emission spectra of FAM
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Cyanine5 maleimide reacts with thiols to give dye labeled conjugates.General properties
| Appearance: | yellow solid |
| Mass spec M+ increment: | 372.07 |
| Molecular weight: | 426.81 |
| CAS number: | 2183440-64-2 |
| Molecular formula: | C21H15N2ClO6 |
| IUPAC name: | Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-dihydroxy-3-oxo-, hydrazide, hydrochloride |
| Solubility: | good in ethanol, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 492 |
| ε, L⋅mol−1⋅cm−1: | 74000 |
| Emission maximum, nm: | 517 |
| Fluorescence quantum yield: | 0.93 |
| CF260: | 0.22 |
| CF280: | 0.17 |
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