Pyrylium-1 (Py-1)

Protein labeling

Cat. #	Quantity	Price	Lead time	Buy this product
4698-1mg	1 mg	$329	in stock

Found better price? Let us know and we will propose the way forward!

Pyrylium-1 (Py-1, Chromeo™ P503) is a fluorogenic amine-reactive dye that is not fluorescent itself but forms a fluorescent product with emission at 600 nm after conjugation with primary amine groups of peptides and proteins.

Pyrylium-1 displays a weak fluorescence with a quantum yield of less than 1% in solution. After conjugation to primary amines, the dye exhibits a color change from blue to red and undergoes a shortwave spectral shift of more than 100 nm, and the quantum yield rises to 50%. The shift of the absorption/emission bands and the increased fluorescence quantum yield significantly eliminate the background from an unbound dye. Also, unbound Pyrylium dyes are hydrolyzed during the labeling procedure. Altogether, these features allow the labeling of amine-containing molecules via a simple one-step, room-temperature incubation without additional purification steps.

Pyrylium-1-labeled peptides and proteins are ready to use immediately after conjugation. They can be used successfully in a number of «no-wash» applications, such as SDS-protein gel electrophoresis, capillary electrophoresis, isoelectric focusing, and as a fluorescent label in receptor binding studies. Proteins labeled with Pyrylium-1 maintain their native charge and isoelectric point.

Download in
ChemDraw format

Excitation and emission spectra of Pyrylium-1 (Py-1)

Recommended protocol

Protein Labeling with Pyrylium Dyes

Calculator

Solution calculator

General properties

Appearance:	deep blue powder
Molecular weight:	393.23
Molecular formula:	C₂₁H₂₄BF₄NO
IUPAC name:	(E)-2,6-dimethyl-4-(2-(2,3,6,7-tetrahydro-1H,5H-pyrido[3,2,1-ij]quinolin-9-yl)vinyl)pyrylium
Solubility:	DMSO, DMF, methanol, acetonitrile
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Spectral properties

Excitation/absorption maximum, nm:	612 nm (free); 503 nm (conjugated)
Emission maximum, nm:	665 nm (free); 600 nm (conjugated)

Chromeo™ is a trademark of Active Motif Chromeon GmbH.

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.