TOR-G4, G-quadruplexes fluorescent probe
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A5510 | 50 uL, 5 mM/DMSO | $149 | in stock | |
| 15510 | 500 uL, 5 mM/DMSO | $390 | in stock | |
| 4357-1g | 1 g | please inquire | in stock |
G-quadruplexes (G4s) are secondary structures that form in DNA and RNA through noncanonical hydrogen bonding between four guanine bases [1,2]. In the nucleus, DNA G4s have been associated with epigenetic regulation of gene expression through their interactions with regulatory proteins, such as transcription factors and chromatin modifiers [3,4]. RNA G4s have been linked to RNA splicing, transport, and translation regulation, as well as RNA-mediated stress responses in the cytoplasm [5-7].
TOR-G4 is a thiazole orange derivative, a newly synthesized G4 fluorescent probe [8]. It is a small-molecule alternative to immunochemistry with G4-specific antibodies. TOR-G4 allows the visualization of G4s based on changes to the fluorescence lifetime of a probe upon nucleic acid binding. The lifetime of TOR-G4 is highest in the presence of G4s and lower for other sequences. Within cells, TOR-G4 is primarily colocalized with RNA in the cytoplasm and nucleoli, making it the first lifetime-based probe validated for exploring the emerging roles of RNA G4s in cell functioning. TOR-G4 is suitable for imaging RNA G4s via FLIM [8].
[1] Trends in Chemistry 2, 123 (2020); [2] Nat Rev Mol Cell Biol 21, 459 (2020); [3] Nucleic Acids Res 49, 8419 (2021); [4] Trends Genet 35, 29 (2019); [5] Nucleic Acids Res 48, 12534 (2020); [6] Trends Biochem Sci 46, 270 (2021); [7] Nucleic Acids Res 49, 5426 (2021); [8] J Am Chem Soc 146, 1009 (2024).
Excitation and emission spectra of TOR-G4 (G4 DNA (c-MYC))
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| Appearance: | black solid |
| Molecular weight: | 607.56 |
| Molecular formula: | C30H30IN3OS |
| Solubility: | good in DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 483; 567 (c-MYC complex) |
| Emission maximum, nm: | 648 (c-MYC complex) |
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