BCECF AM, green fluorescent pH indicator
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 3698-50ug | 50 ug | $16 | 12 days | |
| 3698-1mg | 1 mg | $169 | 5 days |
BCECF AM (2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester) is a cell-permeant, dual-excitation ratiometric fluorescent indicator for measuring changes in the intracellular pH.
Cytosolic esterases hydrolyze BCECF AM to yield BCECF, a polar fluorescein derivative held by cells. BCECF is stable in the cells and has an efflux half-life of over two hours. Intracellular pH is estimated by determining the pH-dependent ratio of emission intensity at 530 nm when the dye is excited at 490 nm vs. the emission intensity when excited at 440 nm. This approach can be carried out using spectrofluorometry or flow cytometry methods.
BCECF AM can also be used to investigate intracellular changes in other ions, including potassium.
Product in action
Live culture of MDCK kidney epithelial cells stained with the fluorescent pH indicator BCECF AM
The pH‑dependent fluorescence excitation spectra of BCECF measured at two wavelengths
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| Appearance: | white powder |
| Molecular weight: | 556.47 |
| CAS number: | 117464-70-7 |
| Molecular formula: | C30H20O11 |
| IUPAC name: | 3',6'-bis[(acetyloxy)methoxy]-5(or 6)-[[(acetyloxy)methoxy]carbonyl]-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 2',7'-bis[(acetyloxy)methyl] ester. |
| Solubility: | DMSO, ethyl acetate, toluene, acetonitrile |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 440; 490 |
| Emission maximum, nm: | 530 |
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