SIMA azide, 6-isomer
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| AE230 | 1 mg | $110 | in stock | |
| BE230 | 5 mg | $180 | in stock | |
| CE230 | 10 mg | $310 | in stock | |
| DE230 | 25 mg |
$410
|
in stock | |
| EE230 | 50 mg |
$695
|
in stock | |
| FE230 | 100 mg |
$1190
|
in stock |
SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.
SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.
Absorption and emission spectra of SIMA
Recommended protocol
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General properties
| Appearance: | orange powder |
| Molecular weight: | 679.52 |
| Molecular formula: | C36H24Cl2N4O6 |
| Solubility: | good in DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 531 |
| ε, L⋅mol−1⋅cm−1: | 92300 |
| Emission maximum, nm: | 555 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.57 |
| CF280: | 0.18 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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