SIMA azide, 6-isomer
Cat. # | Quantity | Price | Lead time | Buy this product |
---|---|---|---|---|
AE230 | 1 mg | $110 | in stock | |
BE230 | 5 mg | $180 | in stock | |
CE230 | 10 mg | $310 | in stock | |
DE230 | 25 mg | $410 | in stock | |
EE230 | 50 mg | $695 | in stock | |
FE230 | 100 mg | $1190 | in stock |
SIMA (dichloro-diphenyl-fluorescein) is a dye with spectral properties similar to HEX but with a higher quantum yield.
SIMA azide is used to produce fluorescently labeled primers and hybridization probes for quantitative PCR. Oligonucleotides with a SIMA label can be easily generated via azide-alkyne cycloaddition between SIMA azide and alkyne-containing oligonucleotide.
Absorption and emission spectra of SIMA
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General properties
Appearance: | orange powder |
Molecular weight: | 679.52 |
Molecular formula: | C36H24Cl2N4O6 |
Solubility: | good in DMSO, DMF |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
MSDS: | Download |
Product specifications |
Spectral properties
Excitation/absorption maximum, nm: | 531 |
ε, L⋅mol−1⋅cm−1: | 92300 |
Emission maximum, nm: | 555 |
Fluorescence quantum yield: | 0.63 |
CF260: | 0.57 |
CF280: | 0.18 |
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