Universal CPG type II, 500A

CPG modifier solid supports Oligonucleotide synthesis
Cat. # Quantity Price Lead time
3219-1g 1 g $45 in stock
3219-10g 10 g $410 in stock
3219-100g 100 g please inquire 21 days
Bulk inquiry
Found better price? Let us know and we will propose the way forward!

The Universal CPG type II, 500A is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.

The Universal CPG type II, 500A is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 120 bases. For oligos up to 120 bases universal support 1000 Å can be used.

Usage

Coupling: Standard conditions for universal CPG.

Deprotection: 2 hours at 80°C or 8 hours at 55°C using concentrated ammonia; 15 minutes at 65°C using AMA mixture, ammonium hydroxide - 40% methylamine (1:1).

Universal CPG type II, 500A, 3219-1G
Universal CPG type II, 500A, 3219-1G
Structure of Universal CPG type II, 500A
Download in
ChemDraw format

Customers also purchased with this product

Copper(II)-TВTA catalytic buffer, 1.5x

Ready-to-use catalytic buffer containing сopper(II) and TBTA ligand. It is suitable for click chemistry modification of nucleic acids and small molecules.

VIC azide, 6-isomer

VIC is an asymmetric xanthene dye with spectral properties similar to HEX and JOE, widely used for labeling PCR probes. This derivative is an azide for dye conjugation by click chemistry.

DusQ® 3 CPG 500

DusQ® 3 is a dark quencher for far red and near infrared dyes like Cyanine5.5. This is a controlled pore glass solid support for the synthesis of oligonucleotides with the quencher attached to 3'-terminus.
Add this product to your cart and
get free express delivery

General properties

Appearance: white powder
Quality control: loading measurement, functional testing in oligo synthesis.
Storage conditions: 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: Download
Product specifications

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonium hydroxide 2 hours at 80 °C or AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 15 minutes at 65 °C
Deprotection conditions: identical to protected nucleobases
Your item has been added. View your cart or proceed to checkout
The count of items is incorrect.