Coumarin 343 azide
This product is discontinued, and no longer available.
We recommend AF 343 (Coumarin)-X-azide as a substitute
This product will be discontinued soon in favor of Coumarin 343 X azide. We may still have some material in stock (please see availability above).
This greenish-blue emitting fluorophore can be used as a FRET donor for FAM. Azide derivative is suitable for click chemistry.
Coumarin 343 absorption and emission spectra
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FAM (fluorescein) azide for сlick chemistry labeling, pure 5-isomer.FAM azide, 6-isomer
FAM (fluorescein) azide, 6-isomer is a commonly used fluorescent dye azide for сlick сhemistry.General properties
| Appearance: | yellow powder |
| Molecular weight: | 441.48 |
| CAS number: | 1807503-82-7 |
| Molecular formula: | C22H27N5O5 |
| Solubility: | Good in organic solvents (DCM, chloroform, DMF, DMSO, MeCN, alcohols), poor in water |
| Quality control: | NMR 1H (95%) TLC, functional testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 437 |
| ε, L⋅mol−1⋅cm−1: | 39000 |
| Emission maximum, nm: | 477 |
| Fluorescence quantum yield: | 0.63 |
| CF260: | 0.29 |
| CF280: | 0.24 |
Product citations
- Musiol-Kroll, E.M.; Zubeil, F.; Schafhauser, T.; Härtner, T.; Kulik, A.; McArthur, J.B.; Koryakina, I.; Wohlleben, W.; Grond, S.; Williams, G.J.; Lee, S.Y.; Weber, T. Polyketide bio-derivatization using the promiscuous acyltransferase KirCII. ACS Synthetic Biology, 2017, 6(3), 421–427. doi: 10.1021/acssynbio.6b00341
- Teske, N.S.; Voigt, J.; Shastri, V.P. Clickable Degradable Aliphatic Polyesters via Copolymerization with Alkyne Epoxy Esters: Synthesis and Postfunctionalization with Organic Dyes. Journal of the American Chemical Society, 2014, 136(29), 10527-10533. doi: 10.1021/ja505629w
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