AF 430 azide
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| A0830 | 1 mg | $125 | in stock | |
| B0830 | 5 mg | $325 | in stock | |
| D0830 | 25 mg |
$950
|
in stock | |
| E0830 | 50 mg |
$1625
|
in stock | |
| F0830 | 100 mg |
$2090
|
in stock |
AF 430 is a fluorescent dye. It can be excited by 405 nm violet laser or 445 nm laser paired, for example, with 510/80 nm bandpass filter.
Azide moiety allows quick and effective labeling and detection of terminal alkynes via a copper-catalyzed click reaction (CuAAC) or of strained cyclooctynes via a copper-free click chemistry reaction (SPAAC). Mild reaction conditions are suitable for most biomolecules, cells, and tissues. The product is water soluble and insensitive to pH changes between pH 4 and pH 10.
Absorption and emission spectra of AF 430
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AF 430 tetrazine
Tetrazine derivative of AF 430 fluorescent dye suitable for in vitro cell labeling.BDP® TMR azide
Borondipyrromethene dye with azido group. Spectral properties are similar to TAMRA dye. Due to long fluorescence lifetime, BDP TMR is especially useful for fluorescence polarization measurements.Ascorbic acid
Ascorbic acid is a reducing agent converting copper(II) into a monovalent form that catalyzes the click reaction.General properties
| Appearance: | yellow solid |
| Molecular weight: | 623.69 |
| Molecular formula: | C25H29N5F3KO6S |
| Solubility: | good in water, DMF, DMSO |
| Quality control: | NMR 1H, HPLC-MS (95%) |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | Download |
| Product specifications |
Spectral properties
| Excitation/absorption maximum, nm: | 430 |
| ε, L⋅mol−1⋅cm−1: | 15955 |
| Emission maximum, nm: | 542 |
| Fluorescence quantum yield: | 0.23 |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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