F-ara-EdU (2’-Deoxy-2’-fluoro-5-ethynyluridine)

Alkynes Cell proliferation Click chemistry

Cat. #	Quantity	Price	Lead time
3438-5mg	5 mg	–	in stock
3438-25mg	25 mg	$375	in stock
3438-100mg	100 mg	$825	in stock
3438-500mg	500 mg	please inquire	in stock

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F-ara-EdU (2’-deoxy-2’-fluoro-5-ethynyluridine) is a synthetic analog of thymidine used to study de novo DNA synthesis and cell proliferation. It is a less cytotoxic alternative for BrdU (5-Bromo-2’-deoxyuridine) and EdU (5-Ethynyl-2’-deoxyuridine).

F-ara-EdU incorporates into replicating DNA during the S-phase of the cell cycle instead of natural thymidine. Metabolic incorporation of F-ara-EdU into DNA can be detected by copper-catalyzed click reaction with fluorescent or biotin-labeled azides.

In contrast to EdU, F-ara-EdU causes little or no cellular arrest or DNA synthesis inhibition. Therefore, F-ara-EdU is ideally suited for pulse-chase experiments aimed at birth-dating DNA in vivo and long-term cell survival estimation.

Structure of F-ara-EdU ((2’S)-2’-Deoxy-2’-fluoro-5-ethynyluridine)

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EdU (5-Ethynyl-2'-deoxyuridine)

EdU is a nucleoside that is incorporated into replicated DNA by cellular enzymes. After it, DNA contained in the cells can be developed by click chemistry reaction with fluorescent dye azides to reveal cell proliferation.

AF 430 azide

Azide derivative of AF 430 for labeling and detecting alkyne-containing biomolecules via сlick reaction.

THPTA ligand

THPTA is a water-soluble ligand for Cu(I)-catalyzed click chemistry. The ligand stabilizes copper in its Cu(I) oxidation state. Due to high aqueous solubility, reactions with this ligand do not require an organic co-solvent.

General properties

Appearance:	off-white powder
Molecular weight:	270.22
CAS number:	95740-26-4
Molecular formula:	C₁₁H₁₁FN₂O₅
Solubility:	in water, DMSO, DMF
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	Download
	Product specifications

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.